Immunohistochemistry and immunocytochemistry : essential methods

Simon Renshaw

Description

Immunohistochemistry and Immunocytochemistry: Essential Methods provides a comprehensive yet easy-to-follow overview of two fundamental techniques used to visualize protein expression in tissues and cells. Designed for both beginners and experienced researchers, this guide explains key concepts such as antibody selection, sample preparation, fixation, antigen retrieval, staining protocols, and imaging. It emphasizes optimized workflows, technical accuracy, and reproducibility—factors essential for generating reliable scientific data.
Readers will also find troubleshooting tips, comparisons of manual versus automated staining, and insights into choosing the right controls and detection systems. Whether used in diagnostic laboratories, research settings, or academic training, this resource serves as an invaluable reference for mastering IHC and ICC techniques and enhancing experimental outcomes.